Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii
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چکیده
منابع مشابه
Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii.
Glyoxylate dehydrogenase (glyoxylate:NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per activ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1984
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2180113